recombinant epiregulin  (R&D Systems)

Journal: Hepatobiliary Surgery and Nutrition

Article Title: EREG-secreting THBS1 + tissue monocytes are recruited by C5a to promote rapid liver regeneration in patients and mice during the ALPPS procedure

doi: 10.21037/hbsn-24-391

Figure Lengend Snippet: Proliferation phenotype of THBS1 + tissue monocytes. (A) t-SNE plot of the distribution of the Mo/Mφ population at ALPPS stage II. Results are color-coded according to the growth factor gene expression level. MP, Mo/Mφ population. (B) Violin and t-SNE plots of the EREG expression in THBS1 + tissue monocytes, i.e., THBS1 + tissue monocytes. (C) Network of hepatocyte receptor interactions (left panel) and bubble plot of monocyte cluster ligand and hepatocyte receptor interactions (right panel). (D) Bubble plot showing the expression of receptors in monocyte clusters. (E) Boxplot representing a comparison of the peak concentrations of EREG in the plasma of patients at ALPPS stages I and II. *, P<0.05. (F) Line chart showing the OD450 values of hepatocytes in the EREG group and control group following CCK-8 incubation. (G) In the liver tissue from the murine model of ALPPS, regeneration was impaired after systemic neutralization of EREG. (H) Survival analysis of EREG rescue versus vehicle treatment for mice with PHLF. *, P<0.05; **, P<0.01; ***, P<0.001 (actual P values were reported in results). ALPPS, associating liver partition and portal vein ligation for staged hepatectomy; CCK-8, Cell Counting Kit-8; EREG, epiregulin; FLR, future liver remnant; Mo, monocytes; Mφ, macrophages; MP, Mo/Mφ population; PHLF, post-hepatectomy liver failure; TM, tissue monocyte; t-SNE, t-distributed stochastic neighbor embedding.

Article Snippet: The cells were resuspended in minimum essential medium (MEM) containing 10% FBS with recombinant EREG (5 μg/mL; rmEpiregulin/CF, 1068-EP-050/CF, R&D Systems), recombinant AREG (5 μg/mL; rmAmphiregulin/CF, 989-AR-100/CF, R&D Systems), recombinant EREG plus recombinant AREG (5 μg/mL), or vehicle control and inoculated into a 96 well plate with 1,000 cells per well.

Techniques: Gene Expression, Expressing, Comparison, Clinical Proteomics, Control, CCK-8 Assay, Incubation, Neutralization, Ligation, Cell Counting

Journal: Hepatobiliary Surgery and Nutrition

Article Title: EREG-secreting THBS1 + tissue monocytes are recruited by C5a to promote rapid liver regeneration in patients and mice during the ALPPS procedure

doi: 10.21037/hbsn-24-391

Figure Lengend Snippet: THBS1 + tissue monocytes were recruited through C5a. (A) Multiplexed immunofluorescence staining, showing the distribution of C5aR1 + monocytes (CD14 + C5aR1 + ). (B) Immunohistochemical staining of C5a at ALPPS stage I (upper left) and stage II (lower left). The H-score shows the up-regulation of C5a expression in hepatocytes from ALPPS stage I to stage II. *, P<0.05. (C) Liver samples from the murine ALPSS model on POD1 and POD5 (left). The boxplot represents comparisons of the FLR to body weight ratio between the control group and PMX-53 group on POD1 and POD5. (D) Immunohistochemical staining of Ki-67 for the control group and PMX-5 group on POD1 and POD5 (left). The boxplot shows comparisons of the ratios of Ki-67 + cells between the control and PMX-53 group on POD1 and POD5 (right). (E) Boxplot showing the EREG expression in the control and PMX-53 group on POD1 and POD5. (F) Schematic diagram of the ALPPS-induced liver regeneration. *, P<0.05 (actual P values were reported in results). ALPPS, associating liver partition and portal vein ligation for staged hepatectomy; EREG, epiregulin; IF, immunofluorescence; IHC, immunohistochemistry; FLR, future liver remnant; POD, post-operation day.

Article Snippet: The cells were resuspended in minimum essential medium (MEM) containing 10% FBS with recombinant EREG (5 μg/mL; rmEpiregulin/CF, 1068-EP-050/CF, R&D Systems), recombinant AREG (5 μg/mL; rmAmphiregulin/CF, 989-AR-100/CF, R&D Systems), recombinant EREG plus recombinant AREG (5 μg/mL), or vehicle control and inoculated into a 96 well plate with 1,000 cells per well.

Techniques: Immunofluorescence, Staining, Immunohistochemical staining, Expressing, Control, Ligation, Immunohistochemistry

Journal: bioRxiv

Article Title: Menin-MLL Inhibitor MI-503 Blocks Menin Nuclear Export and Suppresses Hypergastrinemia

doi: 10.1101/2022.05.17.492246

Figure Lengend Snippet: (A ) Volcano plot of upregulated and down-regulated genes expressed by enteric glial cells treated with gastrin; ( B ) Levels from RNA-Seq plotted as a function of treatment; ( C ) Levels of epiregulin or Neuregulin as a function of gastrin or MI-503 treatment; ( D ) Western blot showing effect of erlotinib (EGF receptor antagonist) on glial cells treated with gastrin.

Article Snippet: Recombinant mouse Epiregulin was purchased from Sino Biological and mouse neuregulin1 from R&D Systems.

Techniques: RNA Sequencing Assay, Western Blot